Comparative Study on Antimicrobial Activity and Microbial Load of Alternanthera philoxeroides (Mart.) Griseb Collected from Polluted and Unpolluted Site

U. Arul Pamila and S. Karpagam

PhD Research Scholar, Department of Botany, Queen Mary's College (A), Chennai, India

Associate Professor, Department of Botany, Queen Mary's College (A), Chennai, India

Accepted 21 November, 2017

Citation: Arul Pamila U, Karpagam S. (2017). Comparative Study on Antimicrobial Activity and Microbial Load of Alternanthera philoxeroides (Mart.) Griseb Collected from Polluted and Unpolluted Site. International Journal of Biological Education 1(1): 002-007.

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Copyright: © 2017 Pamila and Karpagam. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.

Abstract

The present study analyses the antimicrobial activity and the microbial load of an edible plant Alternanthera philoxeroides (Mart.) Griseb growing in polluted and unpolluted site.  The plants were collected and tested against various Gram positive, Gram negative bacteria and fungi. Antimicrobial activity was performed with acetone, aqueous, chloroform, ethanol and petroleum ether extracts of aerial parts A. philoxeroides collected from polluted and unpolluted site that showed significant antimicrobial activity against tested bacterial and fungal organisms.  The extracts were compared with standards like Amoxicillin for antibacterial activity and Ketoconazole for antifungal activity.  The extracts showed remarkable antimicrobial activity as measured from the zone of inhibition and results were comparable with that of standard drugs against the organisms tested. The microbial load is also enumerated in the cooked and cooked refrigerated samples from polluted and unpolluted site.  In conclusion, plant extract of A. philoxeroides collected from polluted site showed less antimicrobial activity and higher antimicrobial activity in unpolluted site.  The ethanol extract showed higher activity when compared to other extracts.  The microbial load is higher in cooked refrigerated sample when compared to cooked sample.

Keywords: Alternanthera philoxeroides, antimicrobial activity and microbial load.