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Ithiru J. Mwangi*
Gichuru E.K
Alakonya A.E
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Ithiru J. Mwangi*
Gichuru E.K
Alakonya A.E
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International Journal of Plant Breeding and Crop Science

Identification of coffee genotypes with resistance to isolates of bacterial blight of coffee in Kenya

Ithiru J. Mwangi*1, Gichuru E.K1, Alakonya A.E2

1Kenya Agricultural and Livestock Research Organization- Coffee Research Institute, P O Box 4-00232 Ruiru, Kenya.

2Institute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000, City Square 00200, Nairobi, Kenya

Accepted 20 September, 2015.

Citation: Ithiru JM, Gichuru EK, Alakonya AE (2016). Identification of coffee genotypes with resistance to Bacterial Blight of Coffee isolates in Kenya. International Journal of Plant Breeding and Crop Science, 3(1): 090-098.

Copyright: © 2016 Ithiru et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.

Abstract

Bacterial Blight of Coffee (BBC) caused by Pseudomonas syringae pathovar garcae is a major concern in Kenya and other countries due to high incidence and severity in Arabica coffee. Previous work to develop disease resistant coffee varieties in Kenya which reduce the cost of production and offer an environmentally safe management did not take resistance to BBC into consideration. The study aimed at identifying coffee genotypes with resistance to selected BBC isolates in Kenya. Twenty four (24) coffee genotypes were screened for resistance using 4 BBC isolates collected from various regions in Kenya. Inoculation was done with a drop of approximately 10 µl of the isolates suspension (109 CFU/ml) using injection method on 4 months old seedlings from potting. Incubation temperature was set at 19-200C and relative humidity maintained at 75%. The experiment was carried out in a Complete Randomized Design. Disease symptoms were scored using a scale of 1 to 5 and the data subjected to analysis of variance and effect declared significant at 5% level. The results revealed significant difference among the coffee genotypes with Rume Sudan being the most resistant. There was no significant virulence effect observed on three isolates but one isolate was different. 

Keywords: Coffea arabica, genotype, Pseudomonas syringae pathovar garcae, isolates.