Neutral genetic diversity preservation in a first-generation breeding population of Guinea pig

Joachim Umba, Alain R. Atangana, Jean-Christophe Kashala, Damase P. Khasa

Institut Supérieur Agro-vétérinaire (ISAV) Saint-Pierre Canisius, P.O. Box 3724 Gombe/Kinshasa, Democratic Republic of the Congo

Centre for Forest Research and Institute for Integrative and Systems Biology, Université Laval, Quebec GIV0A6, Canada.

Faculty of Medicine, University of Lubumbashi, Avenue Likasi, 356, Lubumbashi, Democratic Republic of the Congo

Accepted 29 February, 2017.

Citation: Umba J, Atangana AR, Jean-Christophe K, Khasa DP (2017). Neutral genetic diversity preservation in a first-generation breeding population of Guinea pig. International Journal of Veterinary Science and Animal Husbandry 3(1): 018-027.

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Copyright: © 2017 Umba et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.

Abstract

To increase availability of animal proteins in Democratic Republic of the Congo (DRC), Cavia porcellus was recently enrolled in a breeding program that includes base populations from DRC and Belgium. To preserve whole or part of the source's genetic diversity over breeding cycles, cognizance of genetic diversity in natural populations is required. We tested the cross-amplification of eleven pairs of microsatellite primers that were isolated from Cavia aperea and C. magnea. Amplification tests by polymerase chain reaction were performed on total DNA of 30 C. porcellus using 11 microsatellite loci. All the microsatellites amplified at the expected size and were polymorphic for Cporcellus. Using these microsatellite loci, we assessed the genetic diversity of base populations and of first-generation breeding population. High levels of genetic diversity were found within the base populations. These populations exhibited fixation indices significantly greater than 0, indicating occurrence of inbreeding. Moderate differentiation (RST = 0.123) was observed among the base populations. The first-generation breeding population displayed a significantly lower fixation index (0.083) and a higher genetic diversity (A = 5.73; HO = 0.571; HE = 0.663) than the base populations. Appropriate breeding strategies that would limit inbreeding over breeding cycles are discussed.

Keywords: Cavia porcellus; Diversity; Genetic improvement; Inbreeding level; Microsatellite loci.