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International Research Journal of Biochemistry and Biotechnology
Epidemiology and Characterization of lipophilic yeast (Malassezia) from human dandruff samples using cultural and molecular methods
Sonia Sharma, Sibi G
Department of Microbiology, Indian Academy Degree College-Autonomous, Bengaluru, India
Department of Biotechnology, Indian Academy Degree College-Autonomous, Bengaluru, India
Accepted 25 July 2017
Citation: Sharma S, Sibi G (2017). Epidemiology and Characterization of lipophilic yeast (Malassezia) from human dandruff samples using cultural and molecular methods. International Research Journal of Biochemistry and Biotechnology, 4(1): 068-074.
Copyright: © 2017 Sharma and Sibi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.
Accurate identifications of the species are needed to obtain a better understanding of the role of each individual species in the etiology of disease. Increased number of species under the genus Malassezia, urges to characterize the lipophilic yeasts distribution, dominant species from human dandruff samples. This study aimed at the prevalence of Malassezia species in selected individuals and their identification using morphological, biochemical methods along with molecular characterization. Culture based techniques such as catalase test, tween assimilation, esculin hydrolysis and glycine assimilation were performed and the results revealed that 22 isolates were M. globosa, 12 were M. furfur, 9 were M. sympodialis, 4 were M. obtusa and 3 were M. restricta out of 50 positive samples. PCR-RFLP method was used for the specific identification of the Malassezia isolates. 26S rDNA PCR Products after digestion with CfoI and BstF51 revealed differences between the isolates described the species distribution of Malassezia in dandruff samples to overcome diagnostic limitations.
Keywords: Malassezia, dandruff, lipophilic, PCR-RFLP, 26S rDNA