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Sibi G.
Mritunjay Kumar
Abhimanyu Pratap
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Sibi G.
Mritunjay Kumar
Abhimanyu Pratap
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Journal of Environment and Waste Management

Fruit and Vegetable Waste Hydrolysates as Growth Medium for Higher Biomass and Lipid Production in Chlorella vulgaris

Abhimanyu Pratap, Mritunjay Kumar and Sibi G

Department of Microbiology, Bangalore City College, Bengaluru, India

Department of Biotechnology, Indian Academy Degree College-Autonomous, Bengaluru, India

Accepted 21 July, 2017

Citation: Pratap A, Kumar M, Sibi G (2017) Fruit and Vegetable Waste Hydrolysates as Growth Medium for Higher Biomass and Lipid Production in Chlorella vulgaris. Journal of Environment and Waste Management 4(2): 204-210.

Copyright: © 2017 Pratap et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.


Fruit and vegetable wastes include peels, pulp and seeds that constitute about 40% of the total mass and constitute huge environmental problems. Cultivation of microalgae that utilizes fruit and vegetable wastes as feedstock to produce value added products such as biomass and lipids is a unique approach. Different concentrations of fruit waste hydrolysate (FWH) and vegetable waste hydrolysate (VWH) were used for heterotropic cultivation of Chlorella vulgaris thereby optimizing the suitable hydrolysate concentration for higher biomass and lipid production. FWH in the ratio of 8:2 has produced maximum specific growth rate of 1.92 µ d-1. Higher biomass was recorded in growth medium supplemented with FWH (0.16 mg L-1) than VWH medium. Highest chlorophyll content of 7.2 mg L-1 was observed in 8:2 ratio of FWH whereas it was 4.3 mg L-1 in VWH at the same concentration. Carotenoid content was highest in VWH than FWH media with a maximum content of 0.52 and 0.42 mg L-1 respectively. Fruit waste hydrolysates significantly increased the total lipid content than the vegetable waste hydrolysate medium. Highest lipid content of 6.63 mg L-1 was recorded in 8:2 ratio of FWH. This work demonstrates the feasibility of fruit waste hydrolysate as a nutrient source for algal cultivation and a cost reduction of growth medium in algal biomass and lipid production.

Keywords: Microalgae, fruit wastes, vegetable wastes, hydrolysates, biomass, lipid