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Manish Bhat
Reshma Badi
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Reshma Badi
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World Journal of Microbiology

Isolation studies of Bacteriocin producing Lactic Acid Bacteria from raw goat milk

Reshma Badi and Manish Bhat

Accepted 8 February, 2017.

Citation: Badi R, Bhat M (2017). Isolation studies of Bacteriocin producing Lactic Acid Bacteria from raw goat milk. World Journal of Microbiology, 4(1): 064-071.

Copyright: © 2017 Badi and Bhat. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.

Abstract

Probiotics are live microorganisms which when administered in appropriate amount gives health benefit on the host. In the current study raw goat milk obtained from Belapur village (Belapur, Navi Mumbai, Pin-400614, Maharashtra, India) was the source of isolated lactic acid bacteria. Out of 118 screened isolates, only one isolate i.e. RL 76 showed antimicrobial activity upon primary screening. Optimization of the isolate was carried out to check its bacteriocin producing potential. Isolate grew well at 370C and pH 7. Isolate RL 76 tolerated 2% of bile salt and showed no haemolytic activity. Upon morphological and biochemical tests, the isolate RL76 was identified as Lactobacillus casei. The extracted cell free supernatant was partially purified by ammonium sulphate precipitation. Partially purified cell free supernatant showed antibacterial activity against Staphylococcus aureus, Streptococcus fecalis, Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella typhi and Escherichia coli. The study indicates potential of the isolate as a good probiotic species.

Keywords: Probiotics, Goat milk, Bacteriocin, antibacterial activity, bile salts.