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Roberts MI Eneni
Ilesanmi B Omotayo
*Etukudo M Mbosowo
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Roberts MI Eneni
Ilesanmi B Omotayo
*Etukudo M Mbosowo
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World Research Journal of Agricultural Sciences

Some regulation of In Vitro growth of bush Mango- Irvingia wombolu Mildbr (Irvingiaceae)

*Etukudo M Mbosowo, Roberts MI Eneni and Ilesanmi B Omotayo

Accepted 05 September, 2014.
Citation: Etukudo MM, Roberts EMI, Ilesanmi OB  (2014). Some regulation of In Vitro growth of bush Mango- Irvingia wombolu Mildbr (Irvingiaceae). World Research Journal of Agricultural Sciences 1(2): 007-011.


Copyright: © 2014 Etukudo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.
Abstract

Studies were conducted to regulate in vitro protocols for the establishment of Irvingia wombolu MILDBR. Axillary bud explants of Irvingia wombolu were cultured on various strengths, full (MS + KIN or BAP), half (1/2 MS + KIN or BAP) and one quarter (1/4 MS + KIN or BAP) of MS (Murashige and Skoog) medium supplemented with different levels (0, 1, 2, 3, 4 and 5mg/l) of kinetin (Kin) or benzyl-amino purine (BAP) under aseptic conditions. Subsequently, one quarter strength growth medium with 3mg/l of kinetin (Kin) and 4mg/l of benzyl-amino purine (BAP), which gave the best growth performance was used to study the effects of culture passage (frequency of subculture - 0, 2, 4, 6, and 8 times, subcultured every week) and disinfection time of 0.1% mercuric chloride solution (0, 1, 2, 3, 4, 5, and 6 min) on in vitro establishment of the species. A culture passage of 2 times a week was optimum for regeneration frequency of the species. In addition, a disinfection time of 5 minutes was optimum in terms of reduced contamination and best regeneration frequency for establishment of the species. This study suggests that establishment of Irvingia wombolu explants can be achieved if the basic culture conditions are maintained at optimum levels.

Key Words: Some, Regulation, In vitro, Growth, Irvingia wombolu (Bush mango)